葡聚糖凝胶过滤色谱柱法测定两种氨基酸锌螯合物螯合率的差异性

Chelation Rate of Zinc Amino Acid Chelate Determined by Sephadex Gel Filtration Chromatography

微量元素氨基酸螯合物的螯合率常用来反映微量元素氨基酸螯合物的品质,采用葡聚糖凝胶过滤色谱柱法测定蛋氨酸锌、苏氨酸锌两种氨基酸锌螯合物的螯合率,发现该法测定结果与其他测定方法分析结果一致,表明此法适用于测定蛋氨酸锌的螯合率;但测定苏氨酸锌螯合物的螯合率时,测定结果与其他测定方法分析结果不一致,结合葡聚糖凝胶过滤色谱柱法测定过程,表明此法不适用于测定苏氨酸锌的螯合率。

Chelation rates between trace elements and amino acids are usually used to evaluate chelate quality. In this paper, chelation rates of zinc methioninate and zinc threoninate were determined by Sephadex gel filtration chromatography. Results indicated that Sephadex gel filtration chromatography could be used to determine chelation rate of zinc methioninate due to the consistent result with other determination methods. However, it was not feasible to determine the chelation rate of zinc threoninate.