摘要
基于在酸性条件下Ce(Ⅳ)能氧化抗坏血酸产生微弱化学发光,加入奎宁后能显著增强其发光强度,并且发光强度与抗坏血酸浓度在一定范围内呈线性关系,建立流动注射化学发光分析法测定抗坏血酸。在最佳实验条件下,该法线性范围为0.03～1.0mg/ml,检出限为0.012mg/ml,对0.3mg/ml的抗坏血酸溶液平行测定11次,其相对标准偏差(RSD)为2.5%,可成功应用于VC针剂的测定。
In acidic condition, oxidation of ascorbic acid by Ce （IV） resulted in a poor chemiluminescence, and the addition of quinine exhibited a significant enhancement of luminescence. Furthermore, an excellent linear range between luminescence intensity and ascorbic acid concentration was observed. Thus, a flow injection chemiluminescence analytical method for determining ascorbic acid was developed. Under the optimal condition, the liner range for ascorbic acid was 0.03-1.0 mg/ml with a detection limit of 0.012 mg/ml. The relative standard deviation was 2.5% for 0.3 mg/ml ascorbic acid solution among 11 parallel determinations. Therefore, this proposed method can successfully used to determine quinine injection and tablets.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第24期409-411,共3页
Food Science
基金
陕西理工学院专项科研基金项目(SLG0724)
