婴儿眼分泌物病原菌培养及耐药性分析

Bacterial Culture of Infant Eye Secretions and Drug Resistance Analysis

目的了解婴儿感染性结膜炎和角膜炎病原菌分布情况,并对其耐药性进行分析,为临床治疗提供用药依据。方法对331例眼分泌物进行常规细菌培养鉴定,用K-B法测定病原菌耐药性。结果共分离出副流感嗜血菌、金黄色葡萄球菌、流感嗜血菌、表皮葡萄球菌、真菌等共124株菌,阳性率为37.46%;金黄色葡萄球菌、表皮葡萄球菌和肺炎链球菌对青霉素的耐药率较高,分别为86.36%、88.89%和0;副流感嗜血菌、流感嗜血菌、大肠埃希菌、肺炎克雷伯菌和铜绿假单胞菌对氨苄西林的耐药率分别为12.50%、27.78%、87.50%、100.00%和100.00%;对头孢唑林的耐药率分别为16.67%、16.67%、62.50%、14.29%和100.00%;对复方新诺明的耐药率分别为50.00%、27.78%、12.50%、14.29%和100.00%。结论副流感嗜血菌、金黄色葡萄球菌、流感嗜血菌、表皮葡萄球菌是新生儿眼分泌物中的常见分离菌;对氧氟沙星、环丙沙星等抗菌药物的耐药率较低。

OBJECTIVE To understand the pathogen distribution of infant infectious bacterial conjunctivitis and keratitis,and drug resistance to provide guidence on clinical treatment.METHODS The routine bacterial culture of eye secretions in the 331 cases was identified with K-B to determine the resistance of pathogenic bacteria.RESULTS A total of 124 strains were isolated with positive rate of 37.46%,there were Haemophilus parainfluenzae,Staphylococcus aureus,H.influenzae,S.epidermidis,fungi,etc.The resistance of S.aureus,S.epidermidis and S.pneumoniae to penicillin were 86.36%,88.89% and 0,respectively.The resistance rate of H.parainfluenzae,H.influenzae,Escherichia coli,Klebsiella pneumoniae and Pseudomonas aeruginosa to ampicillin were 12.50%,27.78%,87.50%,100.00% and 100.00%;that to cefazolin were 16.67%,16.67%,62.50%,14.29% and 100.00%;and to cotrimoxazole were 50.00%,27.78%,12.50%,14.29% and 100.00%.CONCLUSIONS The H.parainfluenzae,S.aureus,H.influenzaeand S.epidermidis are isolated from the newborn eye secretions;they were lower resistant to ofloxacin and ciprofloxacin.