新疆多药耐药鲍氏不动杆菌质粒、转座子、整合子遗传标记研究

Genetic Marker Study of Plasmid,Transposon and Integron in Multidrug-resistant Acinetobacter baumannii in Xinjiang

目的为了解新疆地区多药耐药鲍氏不动杆菌(MDR-ABA)的质粒、转座子、Ⅰ类整合子遗传标记存在状况。方法对20株MDR-ABA株进行了质粒遗传标记(traA、traF)、转座子遗传标记(tnpA、tnpU、merA)、Ⅰ类整合子遗传标记(intⅠ1)检测研究。结果20株MDR-ABA tnpU阳性5株,阳性率25%;intⅠ1阳性4株,阳性率20%;其中tnpU与intⅠ1基因同时阳性4株;其他均为阴性。结论Ⅰ类整合子较广泛地存在于新疆地区于鲍氏不动杆菌,多药耐药与细菌携带转座子和整合子相关。

OBJECTIVE To know genetic marker existence condition of plasmid,transposon and integron in multidrug-resistant Acinetobacter baumannii in Xinjiang.METHODS The plasmid（traA,traF）,transposon（tnpA,tnpU,merA） and integron（intⅠ1） in MDR-ABA 20 strains were studied.RESULTS From them 5 strains（25%） were tnpU positiye,4 strains（20%） were intⅠ1 positive,4 strains were tnpU and intⅠ1 simultaneously positive,and others were negative.CONCLUSIONS IntegronⅠexists extensively in A.baumannii in Xinjiang.Their multidrug resistance is correlated with transposon and integron carried by ABA.