全耐药鲍氏不动杆菌的随机扩增DNA多态性分析

Typing of Pandrug-resistant Acinetobacter baumannii in Hospital by Randomly Amplified Polymorphic DNA

目的通过全耐药鲍氏不动杆菌随机扩增DNA多态性(RAPD)基因分型同源性分析,以用于医院感染的追踪控制和流行病学的调查研究。方法收集8个月内从医院分离的15株全耐药鲍氏不动杆菌,提取DNA后进行RAPD分型,同时进行抗菌药物敏感试验和流行病学分析。结果15株全耐药菌株被分为6个RAPD型,其中ICU1I、CU2分别存在两株克隆株,从ICU1转入外科病区的患者,同源性与其完全相同,全敏感菌株与全耐药菌株的同源性差异性很明显。结论ICU存在全耐药鲍氏不动杆菌的局部流行。

OBJECTIVE To study the homology of the pandrug-resistant Acinetobactor baumannii（PRABA） by randomly amplificed polymorphic DNA（CRPD） to guide the control of the nosocomial infection and epidemology investigation.METHODS Eighteen strains of PRABA were collected from Jul 2008 to Mar 2009 in our hospital.The bacteria were examined by DADE BEHRING Microscan WalkAway 96SI;DNA extracted from all isolates was amplified by polymerase chain reaction using random primers.In addition epidemology investigation and antimicrobial susceptibility testing were also under taken.RESULTS Fifteen strains were divided into 6 RAPD profiles.There were two clonal strains derived,respectively,from two intensive care units.One strain was isolated from a patient in surgical ward who was transferred from ICU1,showing same homology with that in the ICU1.The homology was different from sensitive strains and drug-resistant strains obviously.CONCLUSIONS An outbreak of PRABA happens in intensive care unit.