HPLC法测定扶正颗粒中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量

Determintion of 2,3,5,4′-tetrahydroxy stilbene-2-O-β-D-glucoside in Fuzheng Granules by HPLC

目的对扶正颗粒中的主要有效成分2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷,建立含量测定方法。方法采用高效液相色谱法,测定本制剂中二苯乙烯苷的含量。结果 2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量测定线性范围在0.026～0.130mg·mL^(-1),平均回收率为99.32%。结论建立的方法准确、稳定、重现性好,可用于该制剂的质量控制。

OBJECTIVE To establish a method for determination of active principle of 2,3,5,4＇-tetrahydroxy stilbene-2-O-β-D-glucoside in Fuzheng Granules. METHODS 2,3,5,4＇- tetrahydroxy stilbene-2-O-β-D-glucoside in preparation was determined by HPLC. RESULTS Stilbenes showed a linear relationship at the range of 0. 026-0. 130mg·mL^-1. The average recovery was 99.32%. CONCLUSION The method was reliable,accurate and duplication.