青蒿琥酯对肝纤维化小鼠肝细胞内Ca^(2+)分布的影响

Effect of Artesunate on Calcium Ion Distribution in Hepatic Cells of Mice with Hepatic Fibrosis

目的:探讨青蒿琥酯对肝细胞保护作用的机理。方法:采用四氯化碳致小鼠肝损伤模型,利用电镜细胞化学技术和体视学技术研究钙离子在肝细胞的分布情况。结果:模型组钙离子在胞浆中的数密度较正常组和青蒿琥酯高剂量组均有明显增高,差异有非常显著性意义(P<0.01);模型组与青蒿琥酯低剂量组小鼠胞浆钙离子数密度比较,差异无显著性意义(P>0.05);青蒿琥酯高剂量组胞浆钙离子数密度与秋水仙素组比较,差异有显著性意义(P<0.05)。结论:青蒿琥酯具有稳定细胞膜、内质网和线粒体等膜性结构的作用,使影响细胞生理活性的钙离子在胞内外处于稳态的水平,从而阻挡了因钙离子的失衡而启动的脂质过氧化反应对肝细胞的损伤。

Objective:To explore the mechanism of artesunate in preventing hepatic cells.Methods:Mice models of hepatic injury were induced by carbon tetrachloride. Electron microscope cytochemical method and stereological technology were applied for the study of calcium ion distribution in hepatic cells of the model mice. Results:The numerical density of calcium ion in the kytoplasm of hepatic cells was higher in the model group than that in the normal control group and high-dose artesunate group(P<0.01).The difference of numerical density of calcium ion was insignificant between the model group and low-dose artesunate group(P>0.05),but was significant between high-dose artesunate group and colchicines group(P<0.05).Conclusion:Artesuante has an effect on stabilizing cell membrane,endoplasmic reticulum,and mitochondrion of the hepatic cells,which keeps the cells being homeostasis,and inhibits the hepatic injury induced by lipid peroxidation for the imbalance of calcium ion.