壳聚糖／聚乙烯醇／甘油磷酸钠温敏性凝胶的制备及特性研究

Study on Preparation of Temperature Sensitive Hydrogel of Chitosan/Polyvinyl Alcohol/Sodium Glycerophosphate and Its Characteristics

目的:制备具有温敏性并具有一定强度的凝胶。方法:采用壳聚糖(CS)、聚乙烯醇(PVA)和甘油磷酸钠(GP)为基质,以CS的浓度(A)、CS与PVA质量比(B)及pH值(C)为考察因素,以初始凝胶化温度、凝胶强度和脱水情况为指标,采用正交设计优化凝胶处方,并进行验证试验。结果:优化处方为A20mg·mL-1、B1∶1、C7.2。所制凝胶水溶液在4℃和室温下为可流动的液体,于37℃、10min内可发生凝胶化,随温度升高凝胶化所需时间明显缩短,凝胶强度为1.4kPa,凝胶在体外降解过程中28d内pH值几乎不变。结论:CS/PVA/GP凝胶的制备工艺简单可行,并具有温敏性和一定强度。

OBJECTIVE： To prepare temperature sensitive hydrogel which is of strength. METHODS： The hygrogel was prepared using CS, PVA and GP as matrix with concentration of CS （A）, weight ratio of CS to PVA （B） and pH value （C） as factors and with initial gelatination temperature, strength and dehydration as indexes. The formula was optimized by orthogonal experiment and verified. RESULTS： The optimal formula was as follows： A 20 mg,mL , B 1 ： 1, C 7.2. The prepared hydrogel was fluid at 4℃ or room temperature while gelatinized at 37℃ within 10 min with strength of about 1.4 kPa. Gelatination time reduced along with the increase of temperature. The pH value had hardly changed during degradation in vitro within 28 d. CONCLUSION： The CS/PVA/GP hydrogel is simple and practical in preparation technique and is temperature sensitive and of strength.