HPLC法测定聚普瑞锌颗粒中L-肌肽的含量

Determination of L-Carnosine in Polaprezine Granules by HPLC

目的:建立以高效液相色谱法测定聚普瑞锌颗粒中L-肌肽含量的方法。方法:色谱柱为Agilent ODS,流动相为硫代硫酸钠缓冲液(pH3)-乙腈(77∶23),检测波长为215nm,流速为1mL·min-1,柱温为30℃,进样量为10μL。结果:L-肌肽进样量的线性范围为0.1579～3.1584μg(r=1.0000),平均回收率为99.65%,RSD=0.078%。结论:本方法简便、准确、灵敏度高,可用于该制剂的含量测定。

OBJECTIVE： To develop an HPLC method for the determination of L-Carnosine in Polaprezine granules. METHODS： The Chromatographic column was Agilent ODS with detection wavelength of 215 nm and flow rate of 1 mL. min^-1. The mobile phase consisted of sodium subsulfite buffer solution （pH-=- 3） -acetonetrile （77 ： 23）. The column temperature was set at 30 ℃ and injection size was 10 uL. RESULTS：The good linearity was obtained in the range of 0.157 9-3.158 4 ug for L-Carnosine （r= 1.000 0）. The average recovery was 99.65% （RSD=0.078%）. CONCLUSION： The method is simple, accurate and sensitive, and it is suitable for content determination of L-Carnosine in Polaprezine granules.