一点红不同营养器官中鞣质和绿原酸含量测定

Determination of Tannins and Chlorogenic Acid of Different Vegetative Organs in Emilia sonchifolia(Linn.) DC

目的:建立一点红不同营养器官中鞣质和绿原酸含量测定方法。方法:以没食子酸为对照品,采用磷钼钨酸-干酪素比色法测定了鞣质含量;以绿原酸为对照品,采用高效液相色谱法测定了绿原酸含量。结果:没食子酸浓度在2～10mg·L-1范围内,与吸光度呈良好线性关系,平均加样回收率为100.32%,RSD=1.45%,一点红叶、茎、根中鞣质含量分别为0.25%、0.15%、0.22%;绿原酸含量在0.0356～0.5340μg范围内与峰面积积分值呈良好线性关系,平均加样回收率为99.37%,RSD=1.52%,一点红叶、茎、根中绿原酸含量分别为1.28%、1.14%、0.91%。结论:这两种方法灵敏,准确性强,精密度高,重复性好,适用于一点红中鞣质和绿原酸含量的测定。

Objective： To establish the analytical methods for determinating the contents of tannins and chlorogenic acid of different vegetative organs in Emilia sonchifolia （ Linn. ） DC. Methods ： The content of tannins, expressed as gallic acid equivalents, was measured by using the phospho molybdenum tungstic acid - casein calorimetry, and the content of chlorogenic acid, expressed as chlorogenic acid equivalents, was measured by HPLC. Results ： The good linearity between concentrations and absorbances of gallic acid was found in the range of 2 - 10 mg· L^-1, the average recovery was 100. 32% and RSD was 1.45%, the contents of tannins in leaves, stems and roots ofEmilia sonchifolia （Linn.）DC were 0. 25% ,0.15% and 0.22% respectively; The good linearity between content and peak area integral value of chlorogenic acid was found in the range of 0. 0356 - 0.5340μg, the average recovery was 99.37% and RSD was 1.52%, the contents of chlorogenie acid in leaves, stems and roots of Emilia sonchifolia （Linn.）DC were 1.28%, 1.14% and 0.91% respectively. Conclusion： Two proposed methods are sensitive, precise, accurate and reproducible, and they are suitable for the determination of tannins and chlorogenic acid in Emilia sonchifolia （ Linn. ） DC.