HPLC-MS法分析大鼠血浆中灭多威及其代谢产物

Analysis of methomyl and its metabolite in rat plasma by HPLC-MS

目的:目前,国内外还没有大鼠血浆中灭多威及其代谢物研究的完整报道,文中以高效液相色谱-质谱(HPLC-MS)法分析大鼠血浆中灭多威及其代谢产物,为临床检验灭多威中毒提供参考。方法:按照50mg/kg给大鼠灌服灭多威后,大鼠致死,采集第72h心血,固相萃取法提取,HPLC-MS法检测大鼠血浆中灭多威及其代谢物。结果:经与空白组对照发现,给药后大鼠血浆中,除灭多威原体外,还有一种代谢产物,并测得其准分子离子峰及其各级碎片离子。结论:经与对照品比较及质谱断裂规律推断灭多威在大鼠血浆中的代谢产物为S-甲基-N-羟基硫代乙酰亚胺酯。

Objective： No complete study has been reported on methomyl and its metabolite in rat plasma at home and abroad.This study aimed to detect methomyl and its metabolite in rat plasma by high performance liquid chromatography-mass spectrometry（HPLC-MS）.Methods： Ten Wistar rats received intragastric administration of methomyl 50mg/kg,and then executed and kept at room temperature for 72 hours.After that,methomyl and its metabolite in the heart-blood of the rats were determined by solid phase extraction（SPE） and HPLC-MS.Results： Compared with the blank controls,methomyl and its metabolite were detected in the plasma of the experimental rats,and so were protonate ions and multistage fragment ions.Conclusion： The methomyl metabolite in the rat plasma was S-methyl-N-hydroxythioacetamidate.