腺病毒载体纯化及在肿瘤基因治疗中的应用

Purification and application of adenoviral vectors in tumor gene therapy

腺病毒是一种线性无包膜的双链DNA病毒,用于载体构建的主要是人类2型和5型腺病毒。现在通过对腺病毒的改造,已经成功构建出第一代、第二代、第三代和条件复制型腺病毒载体。随着腺病毒载体在蛋白质表达中的广泛使用,对高产量、高纯度腺病毒载体的需求量逐渐增大,因此近年来在传统纯化腺病毒载体的基础上发展出各种纯化新方法。同时,由于腺病毒载体自身的一些优点,使其在疫苗和肿瘤基因治疗中也得到了较为广泛的应用。

Adenoviruses（Ad） are non-enveloped,double-stranded DNA viruses.Ad serotype 2（Ad2） and Ad5 are mainly used to construct Ad vectors（Advs）.The first,second,and third generations of Advs and the conditionally replicating Adv have been successfully constructed.A wide use of the recombinant Adv for protein expression accelerates the requirement of high yields and purity of Advs.Recently various novel purification methods based on traditional methods have been developed.Meanwhile several advantages of Advs have made them extensively applicable in vaccine and tumor gene therapies.