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沙眼衣原体基因CT703在HeLa细胞中的表达 被引量：1

Study on the expression of CT703 mRNA of Chlamydia trachomatis in HeLa cells
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摘要 本文旨在探讨沙眼衣原体(Chlamydia trachomatis,Ct)L2血清型感染HeLa细胞后,在急性感染和持续感染状态下基因CT703mRNA表达水平的变化。透射电子显微镜下确定Ct急性感染和持续感染模型的建立;姬姆萨染色显示,细胞内包涵体体积增大且出现异常增大的网状体;反转录-聚合酶链反应(RT-PCR)检测显示,持续感染状态下Ct基因CT703mRNA表达水平显著低于急性感染状态,推测这可能是Ct在细胞内持续感染的形成机制之一。 The present paper aimed to study the expression of CT703 mRNA of Chlamydia trachomatis （Ct） serovars L2 under acute infection or persistent infection conditions in HeLa cells. Transmission electron microscopy （TEM） was used to determine the establishment of acute infections and persistent infections. The expression of C7703 mRNA in HeLa ceils was detected by reverse transcriptase-polymerase chain reaction （RT-PCR）. Giemsa staining showed that there were enlarged and aberrant reticulate bodies （RBs） in inclusions in persistent infections. The expression of CT703 mRNA in persistent infections was significantly lower than that in acute infections, which may be one of the mechanisms underlying the persistent infection of Ct in cells.
作者 马淑慧 王芙艳 霍治 杜昆 罗奇志 余平
机构地区 中南大学湘雅医学院免疫学系
出处 《微生物与感染》 2009年第4期217-222,共6页 Journal of Microbes and Infections
基金 国家自然科学基金(30600534) 湖南省自然科学基金(08JJ3061)
关键词 沙眼衣原体 CT703 HELA细胞 急性感染 持续感染 Ghlamydia trachomatis CT703 HeLa cells Acute infections Persistent infections
分类号 R759 [医药卫生—皮肤病学与性病学]
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参考文献26

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共引文献8

同被引文献20

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微生物与感染
2009年 第4期

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