菲立磁标记骨髓单个核细胞移植犬心肌梗死区的活体MR示踪

In vivo MR tracking of Feridex labeled bone marrow mononuclear cells in canine myocardial infarction

目的探讨MR活体示踪犬心肌梗死区移植的菲立磁(Feridex)标记骨髓单个核细胞(BM-MNCs)的价值。方法9只杂种雄性犬,手术结扎冠状动脉成功建立8只犬心肌梗死模型,将Feridex体外标记骨髓单个核细胞(BM-MNCs)注射到心肌梗死区。注射后第1、2、4周用1.5T MR行系列扫描。扫描结束后处死动物,细胞移植区组织HE染色及普鲁士蓝染色进行病理分析。结果第1、2周MR扫描发现Feridex标记BM-MNCs注射点T2低信号区,第4周时消失。组织病理学观察于Feridex标记细胞移植区组织HE染色可见新生血管,普鲁士蓝染色易发现聚集的胞浆蓝染的细胞。结论MR能够无创地示踪活体心肌梗死区移植的Feridex标记的BM-MNCs。

Objective To track the magnetically labeled bone marrow mononuclear stem cells（BM-MNCs） in canine myocardial infarction（MI） model with MR.Methods BM-MNCs were labeled with Feridex effectively in vitro and then injected intramyocardially in 8 MI model dogs.Serial MR was performed with 1.5T MR scanner to show the location of the labeled cells compared with histology.Results The injection sites of labeled BM-MNCs could be located on the 1st and 2nd week,but disappeared on the 4th week.Corresponding to these sites,Prussian blue staining consistently showed that large clusters of cells were labeled by dense intracellular iron at the scar tissue.Conclusion Feridex labeling BM-MNCs enables ready detection in the beating heart on a conventional MR scanner after transplantation into canine infarcted myocardium.