LPS对HepG-2细胞因子表达的影响

Effect of LPS on eytokine production by human hepatocellular carcinoma cells HepG-2

目的探讨LPS对HepG-2细胞TGF-β1，VEGF，IL-6表达的影响。方法用LPS刺激HepG-2细胞，RT—PCR和Western-blot方法检测TGF131，VEGF，IL-6基因的转录和蛋白的表达的情况，同时用HTA125阻断TLR4受体检测LPS对HepG-2细胞TGF-β1，VEGF，IL-6基因的转录和蛋白的表达的情况。结果LPS刺激细胞后可以促进TGF-β1、VEGF、IL-6基因的转录和蛋白的表达增加，抑制TLR4受体后LPS对细胞TGF-β1、VEGF、IL-6表达影响下降。结论LPS可以通过TLR4受体来促进HepG2细胞分泌TGF-β1、VEGF、IL-6。

Objective To investigate the production of TFG-β1, VEGF and IL-6 by human hepatoeellular carcinoma cells HepC-2 activated by LPS. Methods The levels of TGF-β1, VEGF, IL-6 mRNA and protein before and after the treatment of LPS were determined by RT-PCR and Westernblot. After inhibition of the TLR4, their levels were determined again. Results The levels of TGF-β1, VEGF, IL-6 mRNA and protein were all upregulated after the treatment of human hepatocellular carcinoma cells HepG-2 by LPS and then downregulated after the inhibition of TLR4. Conclusion As the exogenous ligand of Toll like receptor 4, LPS can promote the production of pro-inflammatory cytokines TGF-β1, VEGF and IL-6 in human hepatoeellular carcinoma ceils.