高糖对大鼠下颌骨成骨细胞葡萄糖摄取的影响

Effect of hyperglycemia on glucose uptake of rat mandibular osteoblasts

目的:探讨高糖对胰岛素诱导的下颌骨成骨细胞葡萄糖摄取和葡萄糖转运蛋白1(glucose transporter1,GLUT1)表达的影响。方法:分离培养大鼠下颌骨成骨细胞,分别予以5.5mmol/L、16.5mmol/L浓度葡萄糖和胰岛素干预,采用氟-18(F-18)标记的脱氧葡萄糖(18F-FDG)测定成骨细胞的葡萄糖摄取,采用Western印迹法检测GLUT1表达水平。采用SPSS13.0软件包对数据进行方差分析和t检验。结果:高糖培养时,成骨细胞的葡萄糖摄取无明显变化,但GLUT1的表达增加35%(P<0.01),胰岛素的应用对葡萄糖摄取无明显影响,但明显使GLUT1的表达下调33%(P<0.01)。结论:高糖能够引起成骨细胞对胰岛素的敏感性下降,诱导成骨细胞产生胰岛素抵抗。

PURPOSE：Growing evidence shows an association between diabetes mellitus and alveolar bone loss.As abnormal cellular glucose uptake and metabolism are partly involved in diabetic complications,this study was undertaken to explore the effect of hyperglycemia on glucose uptake,glucose transporter1 （GLUT1） expression induced by insulin of rat mandibular osteoblasts.METHODS：Primary osteoblasts were isolated and cultured.The cells were incubated for 48h with medium containing 5.5mmol/L and 16.5mmol/L glucose respectively,then treated with or without insulin for 24h.Glucose uptake was detected by using 18F-FDG in the cells and GLUT1 expression levels were evaluated by Western blotting analysis.One-way ANOVA and t test were performed using SPSS13.0 software package.RESULTS：The results showed that with 16.5mmol/L glucose,glucose uptake was not changed significantly,but GLUT1 expression was increased by 35% （P0.01）,insulin had no significant effect on glucose uptake,but it decreased GLUT1 expression by 33% （P 0.01）.CONCLUSIONS：It is concluded that hyperglycemia can induce insulin resistance of osteoblasts,in which alteration in transport activity and function of GLUT1 might be involved.