不同CpG ODN对大鼠骨髓间充质干细胞增殖作用的研究

Effect of Synthesized CpG ODN Sequences on the Proliferation of Rats Bone Marrow Mesenchymal Stem Cells

目的:筛选对大鼠骨髓间充质干细胞增殖具有影响作用的CpG ODN。方法:体外全骨髓贴壁法提取并纯化大鼠骨髓间充质干细胞,传至第3代孵育12 h至完全贴壁,双盲法加入不同CpG ODN共孵育48 h,MTT比色法检测,不同大鼠重复实验3次,筛选促进大鼠骨髓间充质干细胞增殖的CpG ODN。结果:与PBS溶媒对照组相比,有4条CpG ODN对不同大鼠BMSCs均具有显著促增殖作用,MTT结果具有统计学意义(P<0.05)。结论:4条CpG ODN具有显著促进大鼠BMSCs增殖的作用,将在下一步的实验中探讨它们对BMSCs分化功能的作用。

Objective： To screen the optimal CpG ODN for inducing the proliferation of rat bone marrow mesenchymal stem cells （BMSCs）. Methods： The third generation of BMSCs that were separated and purified from rat were plated 12 h before CpG ODNs was added. MTT assay was used to detect cell proliferation after 48h coculture. The above experiments were repeated for 3 times. Results： The mean OD values of four CpG ODNs＇ groups were significantly higher than those in PBS solvent control group and other CpG ODNs groups （P〈0.05 vs all of others）. Conclusion： The four CpG ODN sequences that significantly promote the proliferation of rat BMSCs will be used for the next experiment to explore their effects on the functional differentiation of MSCs.