留兰香提取物体外抗菌作用初步研究

Initial Study on Anti-bacterial Activity of Mentha spicata Linn Extraction in vitro

【目的】研究留兰香提取物的体外抗菌作用。【方法】采用管碟法测定留兰香提取物抑菌圈大小,采用试管2倍稀释法测定留兰香提取物对4种动物病原菌的最低抑菌浓度(MIC),判定抗菌效果。【结果】留兰香水煎剂、醇提物、挥发油对4种动物病原菌有不同程度的抗菌作用,尤其是对猪葡萄球菌抑制效果比较明显,留兰香水煎剂Ⅰ的MIC为31.25mg/ml,醇提物Ⅲ的MIC为62.5mg/ml,挥发油Ⅴ的MIC为2.32mg/ml,挥发油Ⅵ的MIC为2.31mg/ml。【结论】留兰香水煎剂、醇提物及挥发油体外有一定抑菌作用,不同提取方法所得提取物对试验菌抑制效果不同。

To investigate the antibacterial function of Mentha spicata Linn extracts on Escherichia coli、Salmonella、Enterococcus、Staphylococci in vitro.[Method]In the study, the agar Cup-plate method was used to mensurate the size of antibacterial zone of Mentha spicata L. extractions, and the tube doubleness dilution method was used to determine their Minimum inhibitory concentrations （MIC）for 4 animal bacteria strains. [Results]The aqueous extract, the alcohol extract and the volatile oil of Mentha spicata L. has inhibitory effect to the selected strains. All of them have the best inhibitory effect on Staphylococci. The MIC of aqueous extractⅠ（sinking with alcohol ）is 0.0313g/ml. The MIC of alcohol extraction Ⅲ is 0.0625g/ml . The MIC of volatile oil Ⅴ is 2.5μl/ /ml. The MIC of volatile oil Ⅵ is 2.5μl/ /ml.[Conclusion] Mentha spicata L. shows anti-bacterial effects on the experimental strains in vitro. Each kind of mentha spicata extracts has distinctive inhibitory effect to different bacteria.