重瓣长寿花叶片组织培养及植株再生的研究

Study on the Callus Induction and Plantlet Regeneration from the Leaves of Kalanchoe blossfeldiana

通过研究重瓣长寿花的组培方法,为其工厂化生产提供依据。以重瓣长寿花幼嫩叶片为试材,筛选各个培养阶段的培养基配方,研究重瓣长寿花的快速繁殖技术。结果表明,0.1%升汞灭菌4min,外植体污染率为12.5%,死亡率2.5%,效果最佳;愈伤组织诱导培养基以MS+6-BA1.0mg/L+NAA0.1～0.3mg/L最佳,诱导率达95.8%以上;不定芽分化和增殖的最适培养基为1/2MS+6-BA1.0mg/L+NAA0.1mg/L,增殖倍数为12.2;生根培养基用1/2MS+NAA0.5mg/L最好。将泥炭土:蛭石=1:1混合后作为试管苗的移栽基质,幼苗成活率达99%。

The research aimed to provide basis for large-scale production of Kalanchoe blossfeldiana. The young leaves culture and the rapid propagation of Kalanchoe blossfeldiana were carried out and media for various culture stages were selected. The result showed explant sterilized in 0.1% aqueous mercuric chloride for 4 min has best effect, with the contamination rate 12.5%, and the death rate 2.5%. The best inducement culture medium was MS ＋ 6-BA 1.0 rag/L＋ NAA 0.1-0.3 mg/L, and the frequency of callus induction was 95.8%. The suitable culture medium for bud differentiation and proliferation was 1/2MS ＋ 6-BA1.0 mg/L ＋ NAA0.1 mg/L increased 12.2 times. The plantlets are transplanted into nutrient matrix with 99% survival ratio.