施用不同氮源对球等鞭金藻3011生长的影响

The Effect of Different Nitrogen Sources to the Growth Characteristics of Isochrysis galbana Parke

以硝酸钠为氮源作为对照,研究施用氯化铵、硝酸铵和尿素对等鞭金藻3011生长的影响,以寻找最适于等鞭金藻生长的氮源。通过藻细胞密度来探讨不同氮源下等鞭金藻的生长情况,同时分析培养期间的藻液pH。经生物统计分析表明,在培养的前3天实验组和对照组的细胞密度差异性不显著(P>0.05),但从第3天开始,不同组的藻细胞密度开始出现显著差异,表现为以硝酸铵做氮源时对球等鞭金藻3011生长的效果最佳,藻细胞密度显著高于其他氮源,其次为硝酸钠和尿素,氯化铵的效果最差(P<0.05),且一致持续到培养时间结束。对pH的检测结果显示,导致不同氮源培养的藻液生长速率不同的原因并非来自藻液pH的差异。

The study investigated the effect of ammonium chloride, ammonium nitrate and urea on the growth of Isochrysis galbana Parks contrast to sodium nitrate. The indexes that reflect the growth of algae include algae cell density and pH. Statistical analysis showed that the biological in training before 3 days group and control group cell density differences are not significant （P 〉 0.05）, and the first four days later each group of cell density differences are significant （P〈0.05）. The results showed that ammonium nitrate is the best nitrogen source for Isochrysis galbana Parks growth, i.e.algal cell density is significantly higher than that of other source of nitrogen, secondly, sodium nitrate and urea , ammonium chloride in the lowest （P 〈 0.05）.