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胃癌细胞POT1 mRNA表达、端粒酶活性与胃癌发生的关系 被引量：1

Relationship of POT1 mRNA expression and telomerase activity with incidence of gastric carcinoma
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摘要 目的研究人胃癌细胞POT1 mRNA表达、端粒酶活性与胃癌发生的关系。方法采用实时定量荧光PCR分析不同胃黏膜细胞POT1 mRNA的表达,采用TRAP-ELISA半定量分析不同细胞端粒酶活性。结果与癌旁正常黏膜相比,晚期胃癌癌组织POT1 mRNA增高,中重度不典型增生胃黏膜与其正常胃黏膜比较其POT1 mRNA表达也明显增高,肠上皮化生胃黏膜及其正常胃黏膜POT1 mRNA表达无明显差异。晚期胃癌及中重度不典型增生胃黏膜上皮中端粒酶活性明显高于肠上皮化生组织和正常胃黏膜上皮,但是胃癌和中重度不典型增生及肠上皮化生组织和正常胃黏膜间端粒酶活性无明显变化。结论尽管胃癌细胞和中重度不典型增生端粒酶活性高于正常和肠上皮化生胃黏膜细胞,但是POT1 mRNA的表达与之无关。 Objective To study of relationship of the mRNA expression of protection of telomeres 1 （ POT1 ） and the activity of telomerase with the incidence of gastric carcinoma. Methods Lesioned gastric mucosal tissues and the corresponding normal tissues from 30 cases of gastric cancer, 30 cases of moderate or severe atypical hyperplasia, and 30 cases of intestinal metaplasia were collected in this study. The expression of POT1 mRNA was detected with real time-PCR and the activity of telomerase was examed with TRAP-ELISA. Results Real time-PCR analysis showed that POT1 expression was increased in the gastric carcinoma tissues compared with the paracancerous normal mucosal tissues, increased in the severe atypical hyperplasia than in the corresponding normal tissues, but there was no difference between intestinal metaplasia tissues and normal tissues. The activity of telomerase in the advanced gastric tumors and severe atypical hyperplasia was signifi cantly higher than that in intestinal metaplasia tissues and normal gastric mucosal tissues. However no obvious difference was found in the activity in gastric tumors and severe atypical hyperplasia with intestinal metaplasia tissues and normal gastric mucosal tissues. Conclusion Although the activity of telomerase in gastric cancer and severe atypical hyperplasia is significantly higher than that in normal and intestinal metaplasia tissues, POT1 mRNA has no relationship with this change.
作者 王雷 房殿春
机构地区 第三军医大学西南医院全军消化病研究所
出处 《第三军医大学学报》 CAS CSCD 北大核心 2010年第1期74-76,共3页 Journal of Third Military Medical University
关键词 POT1 MRNA 端粒酶活性 胃癌 protection of telomeres 1 telomerase, activity gastric carcinoma
分类号 R735.2 [医药卫生—肿瘤] R345.5 [医药卫生—基础医学]
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参考文献17

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同被引文献23

  • 1沈诚,范士志,陈建明,李志平,李寒.二氮嗪预处理对缺血再灌注大鼠心肌NF-κB和caspase-3表达的影响[J].第三军医大学学报,2007,29(9):789-791. 被引量：1
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第三军医大学学报
2010年 第1期

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