SKOV3卵巢癌细胞中雌激素对LRP16的调控作用及其影响

E2 regulation on LRP16 and its action in ovarian cancer cell lines

目的:研究雌激素受体ER阳性的卵巢癌细胞系中,E2对LRP16的调控作用以及LRP16对细胞增殖的影响。方法:Northern Blot、Western Blot方法分别检测RNA、蛋白表达水平;生长曲线测定过表达或抑表达LRP16对SKOV3细胞生长特性的影响。结果:雌激素敏感的BG-1细胞系中,E2正调控LRP16的表达;而不同浓度雌激素处理雌激素不敏感SKOV3细胞,LRP16蛋白的表达下降,而ICI182780对其作用相反;LRP16对SKOV3细胞的生长、增殖有微弱的调节作用。结论:在雌激素不敏感的SKOV3细胞系中,E2对LRP16的调节作用及LRP16发挥的生长调节作用与雌激素敏感的BG-1卵巢癌细胞系及乳腺癌体外研究结果不同。提示雌激素不敏感浆液性卵巢癌中E2对LRP16的调控可能存在新的机制,可能用来解释部分卵巢癌对内分泌治疗敏感性不同的原因。

Objective：To investigate the regulation of estradiol （E2） on LRP16 and its effect of LRP16 on ERalpha（ ＋ ） ovarian cancer cell line. Methods：Northern Blot and Western Blot examined the expression of LRP16 RNA and protein. The growth trait of cells transfected with LRP16 or LRP16 - siRNA was examined by growth curves. Results： In estrogen- sensitive BG- 1 cells, E2 upregulated LRP16 expression. However in estrogen- insensitive SKOV3 cell line treated with different amount of E2, estrogen decrease LRP16 expression ,ICI182 780 played inversely. LRP16 had slightly effect on the growth and proliferation of SKOV3 cell line. Conclusion：In estrogen -insenstive SKOV3 cell lines ,the regulation of E2 on LRP16 and the function of LRP16 on the growth of SKOV3 were different from the results in BG - 1 ovarian cancer cell line and breast cancer in vitro. It＇s hinted that in estrogen - insensitive serous ovarian cancer new mechanism may exist on E2 regulation to LRP16, which may explain the insensitivity of certain ovarian cancer patients to endocrine therapy.