广西原发性肝癌高危人群中氧化及抗氧化水平分析

Analysis of oxidative and antioxidative levels in blood of a high-risk population of liver cancer

目的:探寻DNA氧化损伤及抗氧化作用在肝癌高危人群中的关系。方法:利用高效液相色谱法检测肝癌高危人群尿中8-OHdG和血清维生素A、E水平,分析其相互关系。结果:广西肝癌高危人群中尿8-OHdG水平明显高于普通人群,男性水平低于女性(P<0.0001);血清维生素A、E的水平属于我国人群的正常值范围;男性血清维生素A水平明显高于女性(P<0.0001);男性维生素E水平明显低于女性(P<0.0001);尿8-OHdG水平与血清维生素A水平呈显著负相关关系(r=-0.10,P=0.015)。结论:DNA的氧化损伤与肝癌的发生发展有密切关系;抗氧化剂的摄入可以对抗机体中氧化代谢产物诱发的氧化损伤。

Objective ：To investigate the role of oxidative DNA damage and antioxidants in high -risk individuals of liver cancer. Methods： Urinary 8 - OHdG, serum Vitamin A, E were measured in bio - samples by HPLC. Resuits： In these high - risk individuals, overall urinary 8 - OHdG levels were higher than in normal population, and significantly lower in males than females （P 〈 0. 0001 ）. Serum vitamin A, E levels were in the normal range of Chinese population, and serum vitamin A levels in males were significantly higher than in females （P 〈 0.0001 ）. Serum vitamin E levels in males were significantly lower than in females（ P 〈 0.0001 ）. Meanwhile, there was a significant negative correlation between urinary 8 -OHdG levels and serum vitamin A levels （r = -0.10,P =0.015）. Conclusion ： Oxidative DNA damage was proved to play a role in hepatocarcinogenesis. Dietary antioxidants reduced oxidative stress in high - risk individuals, and consequently decreased cancer risk of individuals in this special endemic area.