抗 SMV 栽培大豆种质资源的 SCAR 标记指纹图谱分析

SCAR Fingerprinting of Cultivated Soybean Accessions with Resistance to SMV

依据与ＳＭＶ（ＳｏｙｂｅａｎＭｏｓａｉｃＶｉｒｕｓ）抗性基因紧密连锁的共显性ＳＣＡＲ标记ＳＣＷ—０５６６０的序列测定结果，合成了两对ＳＣＡＲ（ＳｅｑｕｅｎｃｅＣｈａｒａｃｔｅｒｉｚｅｄＡｍｐｌｉｆｉｅｄＲｅ－ｇｉｏｎｓ）标记特异引物，对３０个栽培大豆品种进行了指纹图谱分析。结果表明，较短的片段Ｓ—５６００和Ｓ—０５６６０是抗病品种的特征性条带；而较长的片段Ｓ—５１０００和Ｓ—５１６００是感病品种的特征性条带。Ｓｏｕｔｈｅｒｎ杂交结果表明，这两对引物扩增出的条带具有同源性。

Based on sequencing analyses of a codominant SCAR marker SCW 05 660 tightly linked to SMV resistance gene Rsa, two pairs of specific SCAR primers were designed and synthesized to amplify genomic DNA from 30 cultivated soybean accessions. Fingerprinting results showed that shorter fragments S 5 600 and S 05 660 were typical bands in disease resistance varieties, while longer fragments S 5 1000 and S 5 1600 were typical bands in susceptible accessions. Southern blot hybridization results showed that these fragments generated by the two pairs of SCAR primers were homologous.