摘要
在对新疆、内蒙干旱地区采集的48株苜蓿、草木樨、锦鸡儿根瘤菌进行数值分类和SDS-PAGE全细胞蛋白分析的基础上,对数值分类确立的三个亚群进行了16SrDNA-RFLP分析,在87%的相似性水平上,三个新亚群的菌株相应聚类成群。其中第2亚群属于Sinorhizobium分枝,与S.meliloti,S.fredi.S.teranga,S.Saheli在92%的相似水平上聚在一起。第3亚群则与Rhizobium处于同一分枝且与属内各已知种相互区分开,可能为一独立的种。第1亚群有可能为一独立的属,值得进一步研究。此结果进一步证实来自干旱地区的根瘤菌具有很大特异性。
Based on the results of the numerical taxonomy for 48 strains isolated from arid region in Xinjiang and Inner Mongolia, 16S rDNA RFLP is applied to make sure the phylogenetic position of three new subgroups in numerical taxonomy. Above the similarity level of 87%, three new subgroups are clustered into the different subgroups respectively. Subgroup 2 is clustered into Sinorhizobium branch with S.meliloti S.fredii. S. teranga. S.saheli above the similarity level of 92%; Subgroup 3 is located in Rhizobium branch, and separated from the known species in Rhizobium; Subgroup 1 is different from the known genera. These results show particularities of Rhizobia isolated from arid regions in Xinjiang and Inner Mongolia in advance.
出处
《高技术通讯》
EI
CAS
CSCD
1998年第9期50-54,共5页
Chinese High Technology Letters
基金
国家自然科学基金