黄连解毒汤提取动态过程及沉淀产生机制的初步研究

Preliminary study to investigate dynamic extract process of Huanglian Jiedu Tang and the mechanism of subsidence produce

目的:运用统计学方法,初步探讨黄连解毒汤提取动态过程中"沉淀物"的产生及机制。方法:采用UV测定其中总生物碱、总黄酮的量,HPLC同时测定小檗碱、药根碱、巴马汀、黄芩苷、栀子苷的量并计算固含率,沉淀率。采用SPSS统计软件评价各指标性成分、沉淀率、固含率之间的相关性。结果:总生物碱中的小檗碱等的量均与总生物碱呈较好的相关性。总黄酮中黄芩苷与总黄酮的相关系数并不高,而总黄酮与栀子苷的相关系数略高;沉淀率与各指标性成分不显示有极高的相关性。固含率与各指标性成分的相关系数相对较大。结论:随提取时间的变化,提取液中以小檗碱为代表的总生物碱及黄芩苷等达到一定浓度后可视为一平衡点或称为沉淀生成的临界点,即可发生反应,生成"沉淀物";由于"沉淀物"的生成,降低了上述成分浓度,破坏了"平衡状态"。药材中相关成分溶出速度增加,同时沉淀物可能部分溶解,达到新的平衡状态。

Objective： To study the phenomenon of subsidence emergence in the process of Huanglian Jiedu Tang decoction extraction, and the mechanism of subsidence emergence. Method： UV was applied to determine the concentration of total alkaloids and total flavones ;Simultaneous determination of berberine ,jatrorrhizine ,palmatine, baicalin and geniposide were carried out by HPLC ;The solid holdup and precipitation rate were calculated by the formula. The relativity among these parameters was analyzed by the SPSS soft- ware program. Result： The contains of berberine, jatrorrhizine, palmatine in total alkaloids showed a good correlation with total alka- loids. The correlation between baicalin and total flavones was lower than that between geniposide and total flavones. Compared to pre- cipitation rate, solided hold up shows a larger relevance with index component. Conclusion： With the change of time, the total alkaloids represented by berberine alkaloids and baicalin at a certain concentration can be regarded as the equilibrium point, or one generated by the critical point of precipitation ,the reaction can generate ＂sediments＂; because ＂precipitation objects＂ generated, reducing the concentration of the above-mentioned components, destroy the＂ balance＂. The relevant components of herbal medicine increased dissolution rate, at the same time might partly dissolved sediment, reaching a new equilibrium state.