隐丹参酮对宫颈癌Hela细胞增殖及细胞凋亡的影响

Effects of cryptotanshinone on proliferation and apoptosis of Hela cell line of cervical cancer

目的：研究隐丹参酮对人宫颈癌Hela细胞生长的抑制作用及对细胞周期和凋亡的影响。方法：MTT法检测不同浓度隐丹参酮分别存24，48，72h对Hela细胞的抑制作用；流式细胞仪（FCM）检测隐丹参酮对Hela细胞周期和凋亡的影响；Westernblot法检测E6，p53，p21蛋白的表达。结果：不同质量浓度（0．5～16mg·L-1）隐丹参酮对Hela细胞的毒性均有明显剂量和时间依赖性，其24，48，72h的IC50值分别为17．8，8．17，6．55mg·L-1；隐丹参酮可使Hela细胞细胞周期的构成发牛明显的变化并诱导细胞凋亡。Westernblot表明，隐丹参酮作用Hela细胞后HPVE6的蛋白水平表达逐渐降低，p53和p21的蛋白水平表达逐渐升高。结论：隐丹参酮对宫颈癌Hela细胞有较强的细胞毒性，其作用机制可能是使Hela细胞生长停滞舟G0／G1期并诱导细胞凋亡，使s期细胞比例降低；抑制HPVE6的蛋白的表达，使p53功能恢复，启动凋亡，从而起到杀伤肿瘤的作用。

Objective： To investigate the proliferation effects and apoptosis induction of cryptotanshinone on Hela cell line of cervical cancer. Method： The MTT assay was used to detect the growth inhibition rates of Hela cells at 24, 48, 72 h which cultured with cryptotanshinone in different concentrations. The cell cycle distribution and apoptosis were measured by flow cytometry. The protein expressions of E6, p53 and p21 were studied by Western blot. Result： The different concentrations （0. 5-16 mg . L-1） of cryptotanshinone had cytotoxicity on Hela cells, which were clearly dose and time-dependent. The IC50 of 24, 48, 72 h were 17.8, 8.17, 6. 55 mg.L-1, respectively. Cells were treated with cryptotanshinone which had significant effects on cell cycle of Hela cell, and induced apoptosis. Western blot showed cryptotanshiuone decreased expressions of HPV E6 and increased expressions of p53 and p21 proteins. Conclusion： Cryptotanshinone had significant cytotoic and radiosensitization effects on cervical cancer Hela ceils. One of the mechanism may be that it might make significant G0/G1 phase arrest and induced apoptosis, a decrease in S phase, and restore the function of p53 to induce apoptosis in Hela cells to kill the tumor cell.