超微牛煅烧骨的制备、表征和生物相容性评价

Preparation,analysis of properties and evaluation of biocompatibility of ultrafine bovine true bone ceramic

以天然牛骨为原材料,采用化学法脱除有机成分,经过煅烧、超微粉碎制备了超微牛煅烧骨(TrueBoneCeramic,TBC)。对超微粉碎前后和不同煅烧温度、煅烧时间条件下的超微TBC进行了扫描电镜(SEM)测试和X射线衍射(XRD)分析,结果表明,温度越高,TBC颗粒粒径越小,同时超微粉碎后TBC颗粒粒径分布更均匀。也证明了制备的超微牛煅烧骨的成分是羟基磷灰石(Hydroxyapatite,HA),对制备的超微TBC进行了溶血实验、全身急性毒性实验,结果表明,制备的超微牛煅烧骨具有良好的生物相容性。

The ultra-fine bovine true bone ceramic（TBC） was prepared by calcining the raw bovine bone after getting rid of organic component by chemical method, and the TBC particles were smashed and ultrafine ground finally.Then, the ultra-fine bovine TBC prepared under different calcined temperature and times were examined by SEM and XRD, also, the powder before and after ultrafine grinding.The results showed that with the increasing of calcined temperature, the diameter of TBC particles decreased, and the distribution of ultra-fine bovine TBC particles would distributed more homogenously.Also, the component of the ultra-fine bovine TBC was proved to be HA according to XRD results. At last, the biocompatibility was evaluated by detecting the blood resolving rate and overall emergency toxicology, and the ultra- fine bovine TBC particles were proved to have good biocompatibility.