摘要
本文将脲酶通过戊二醛的偶联固定在多孔玻璃珠上。固定化脲酶的活力回收和稳定性良好。在固定化脲酶柱上,脲素定量转化为氨,与酚一次氯酸反应,用分光光度计测定。脲素的回收率>92％,变异系数<4％,最低检出限为3×10^(-6)mol/L。
The present paper describes the urease covalently bounded to controlled pore glass via glutaraldehyde coupling. The recovery of the enzyme activity and the stability of immobilized urease are studied. The recovery of the added urea is nearly 92% and the variant cofficient is less than 4%. The concentration of urea as low as 3×10^(-6) mol/L can be detected by the method.
出处
《南京大学学报（自然科学版）》
CAS
CSCD
1990年第3期437-441,共5页
Journal of Nanjing University（Natural Science）
