PRL-3基因在前列腺癌组织中的表达分析

Expression and Analysis of PRL-3 in Prostatic Carcinoma Tissues

检测PRL-3在前列腺癌中的表达,探讨其与前列腺癌发生发展的关系。Westernblot、RT-PCR检测52例前列腺癌组织及其对应癌旁增生组织,12例阳性淋巴结相对表达水平,并分析其表达水平与临床病理学指标的关系。结果52例前列腺癌中PRL-3基因表达量较相应癌旁增生组织高,差异有统计学意义(p<0.05),12例淋巴结转移灶PRL-3表达量明显高于原发肿瘤、阴性淋巴结及癌旁增生组织(p<0.01)。PRL-3基因的表达水平与前列腺癌分化程度、淋巴结转移有关(p<0.05),与年龄、家族史、血清PSA含量无关(p>0.05)。说明PRL-3的表达与前列腺癌发生发展关系密切,PRL-3可作为前列腺癌诊断、治疗及预后判断过程中的一种较有价值的指标。

To detect the expression of PRL-3 relationship with tumor occurrence and metastasis in prostatic carcinoma tissues. RT-PCR and Western blot were used to detect the expression of PRL-3 in prostatic carcinoma tissues and paired paratumor normal tissues from 52 PCA patients,metastatic lymph nodes from 12 cases with metastasis. It is resulted that PRL-3 level was increased in primary PCA tissues as compared with paired paratumor normal tissues （P 〈 0.05）. However,the PRL-3 levels of metastatic lymph nodes in 12 cases were significantly high compared with the matched primary lesions,negative-lymph nodes and normal tissues （P 〈 0.01 ）. There was significant relation of the expression of PRL-3 with the clinicopathological features including degree of differentiation and metastatic （P 〈 0.05 ）,but no relation with age,family medical history,PSA was found （P 〉 0.05） . It is conclused that of PCA,strongly suggesting that the leve PRL-3 gene plays an important role in metastasis and carcinogenesis of PRL-3 can be used to indicate the potential of metastasis