中药散血明目片抑制兔视网膜静脉阻塞后视网膜VEGF和bFGF的表达

Experimental study of sanxuemingmu tablet on protecting retina after retinal vein occlusion

目的:探讨活血通脉利水明目作用的中药散血明目片防治视网膜静脉阻塞(retinal vein occlusion,RVO)并发新生血管的作用。方法:将家兔48只96眼随机分为4组,每组12只24眼。A健康空白组;B模型组;C血栓通组;D散血明目片组。采用激光光凝法建立兔RVO模型,分别于术后3,7,14,21d用空气栓塞法处死动物,即刻摘取眼球石蜡包埋切片。免疫组化法观察兔视网膜组织中血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)的表达。结果:成功建立了RVO模型。D组与B组比较,视网膜组织中VEGF含量(21d时分别为0.442±0.034和0.583±0.056)和bFGF含量(21d时分别为0.419±0.040和0.514±0.056)明显减少,差异有统计学意义(P<0.05),与C组比较差异无统计学意义。结论:活血通脉利水明目之散血明目片能抑制视网膜面VEGF和bFGF的高表达。

AIM： To investigate the effects of sanxuemingmu tablet（SXMMT） that can activate blood, relieve meridians, eliminate dampness and dissipate stagnation on the treatment of retinal vein occlusion（RVO） and the action mechanism of preventing the new blood vessel. METHODS： Forty-eight rabbits （96 eyes） were randomly divided into 4 groups, with 12 （24 eyes） in each one. Group A was normal group, group B was modeling group, group C was xueshuantong group and D was SXMMT group. RVO models were made by laser photocoagulation. Rabbits were killed in the 3^rd,7^th, 14^th and 21^st day after the operation. Then eyeballs were taken, dehydrated and embedded in paraffin to observe the expression of VEGF and bFGF in the retina by immunohistochemistry. RESULTS： The RVO models of group B,C,D were successfully established. Compared with B group, the content of VEGF and bFGF of group D were obviously reduced, and had significant difference. CONCLUSION： SXMMT could promote absorbing blood after RVO, improve retina hypoxic-ischemic conditions, depress the excessive expression of VEGF and bFGF in retina surface after RVO, treat RVO and control new vessel, and then protect the retina.