配戴软性角膜接触镜者ICAM-1和HLA-DR表达的改变

Changes of ICAM-1 and HLA-DR induced by long-term soft contact lens wear

目的:观察正常人和配戴软性角膜接触镜(soft contact lens,SCL)人群结膜上皮细胞表面亚临床炎症因子HLA-DR和细胞内黏性分子1型ICAM-1(CD54)的表达改变。方法:采用结膜印迹细胞学(conjunctivali mpression cytology,CIC)收集结膜上皮细胞,流式细胞学检查方法测定正常人及配戴软性角膜接触镜者HLA-DR和CD54的表达水平,计算阳性细胞率。结果:长期配戴软性角膜接触镜(>5a)组及(<5a)组中ICAM-1和HLA-DR的表达较正常人组相比明显增加(PHLADR1<0.01,PICAM-1,1<0.01;PHLADR2<0.01,PICAM-1,2<0.01),,前二者比较CD54有明显变化(P<0.01)而HLA-DR无明显变化(P>0.05)。结论:长期配戴软性角膜接触镜者结膜亚临床炎症因子ICAM-1和HLA-DR明显增加,眼表的亚临床炎症增加。

AIM： To observe the change of inflammation agent production of the conjunctiva HLA-DR and intercellular adhesion molecule type 1 （ICAM-1） （CD54） in normal subjects and in soft contact lens （SCL） wear subjects. METHODS： Conjunctival impression cytology（CIC） were used to collect conjunctival epithelium, and the expression levels of HLA-DR and CD54 in normal subjects and in SCL wear subjects were determined by flow cytometry. The percentage of positive cells was calculated and compared between each group. RESULTS： A significant increase of inflammation markers （HLA-DR and ICAM-1） was observed in the long-term（〉5a） and short-term（〈5a） SCL group compared with the control group （PHLADR1〈0.01,PICAM-1,1〈0.01;PHLADR2〈0.01,PICAM-1,2〈0.01）. For the two SCL groups, ICAM-1 has significant change（P〈0.01）, but HLA-DR has no significant change（P〉0.05 ）. CONCLUSION： This study confirms the difference in expression of inflammation markers （HLA-DR and ICAM-1） in SCL and the healthy adult. It demonstrates a significant increase of subclinical conjunctival inflammation in SCL compared with the normal.