改良甘油长期冷冻保存角膜的超微结构及板层角膜移植

The ultrastructure of corneas preserved by long-term cryopreservation with improved glycerol and lamellar keratoplasty

目的:研究改良甘油长期冷冻保存角膜的超微结构及板层角膜移植。方法:将涂有透明质酸钠(sodium hyaluronate,SH)的角膜片在-45℃以下长期甘油低温冷冻保存,保存12mo之后,用于15例板层角膜移植并进行透射电镜检查。结果:15例手术均顺利,术后随访6～24mo,13例植片透明,1例植片呈半透明状,1例植片部分新生血管化。透射电镜检查显示角膜超微结构保存良好。结论:甘油长期冷冻保存角膜有一定活性,适合应用板层角膜移植。

AIM： To study the ultrastructure of comeas preserved by long-term cryopreservation with improved glycerol and to study the lamellar keratoplasty. METHODS： The corneas coated with sodium hyaluro-nate were long-term preserved in -45 ℃ glycerol. After 1 year, 15 cases accepted lamellar keratoplasty with the long-term preserved cornea, and the cornea taken trans-mission electron microscopy（TEM） examination. RESULTS： All surgeries were successful, and patients were followed up for 6 to 24 months. 13 grafts were transparent, 1 graft was translucent, and 1 graft was neovascularization. TEM examination showed that the ultrastructure of cornea was well-preserved. CONCLUSION： The improved long-term cryopreserva-tion with glycerol could preserve some viability of cornea. It is a proper way of preservation that could be used for lamellar keratoplasty in clinic when there is no fresh corneas.