粉防己碱诱导宫颈癌细胞凋亡的定性定量研究

Qualitative and quantitative study on the apoptosis of cervical cancer cells treated by tetrandrine

目的从定性定量角度探讨粉防己碱诱导宫颈癌HeLa细胞凋亡的作用。方法采用MTT法检测不同浓度粉防己碱作用不同时间对人宫颈癌HeLa细胞株的增殖抑制作用。通过流式细胞仪和激光共聚焦显微镜检测细胞凋亡情况。结果粉防己碱对宫颈癌HeLa细胞具有增殖抑制作用,且呈时间、浓度依赖性。应用流式细胞仪检测到15μmol/L粉防己碱诱导HeLa细胞产生的凋亡率为(51.8±0.97)%,显著高于对照组凋亡率(24.3±1.23)%(P<0.05)。通过激光共聚焦显微镜观察,与对照组比较,粉防己碱作用后HeLa细胞具有明显的凋亡形态。结论粉防己碱能够抑制HeLa细胞的增殖并诱导其凋亡。

Objective To study the effects of tetrandrine on apoptosis of HeLa cervical cancer cells qualitati.vely and quantitatively. Methods We measured tetrandrine-induced inhibition of HeLa cell proliferation at different concentrations and time points by MTT assay. The rate of Hela cell apoptosis induced by tetrandrine was detected by flow cytometer and confocal laser scanning microscope （CLSM）. Results Tetrandrine inhibited the proliferation of HeLa cells in dosage- and time-dependent manners. Flow cytometry showed that the apoptosis rate was （51.8 ±0.97）% at the concentration of 15 μmol/L, which was significantly higher than that in the control group （24.3 ± 1.23）% （P〈0.05）. The cells treated with tetrandrine showed typical apoptotic morphology under CLSM. Conclusion Tetrandrine can inhibit proliferation and induce apoptosis of HeLa cervical cancer cells.