隆朋麻醉对犬红细胞免疫黏附性及红细胞膜流动性的影响

Effects of Xylazine on Erythrocyte Adhesion and Membrane Fluidity in Dogs

本试验旨在研究隆朋麻醉对犬红细胞黏附性和膜流动性的影响。试验选用健康本地犬12只,肌肉注射隆朋3.0mg/kg,于麻醉前（即0 h对照组）、麻醉后2、8、24、72、120、168 h动态采集5 mL外周静脉血,离心分离红细胞,制取红细胞悬液并制备试验用红细胞膜,进行C3b受体花环率和免疫复合物花环率的检测及红细胞膜流动性的检测。C3b受体花环率在麻醉后2 h出现下降趋势,但与对照组相比差异不显著（P〉0.05）,注射隆朋后8 h低于对照组且差异极显著（P〈0.01）,此后开始恢复,到麻醉后168 h基本恢复正常,与对照值相比差异不显著（P〉0.05）。而免疫复合物花环率则呈现相反的趋势,其在注射隆朋后出现先上升后下降的趋势。免疫复合物花环率在注药后2-24 h高于对照组且差异极显著（P〈0.01）,72 h基本恢复,与对照组相比差异不显著（P〉0.05）。使用荧光偏振法测定红细胞膜的流动性,结果表明,注射隆朋后犬红细胞膜流动性增加,但与对照组相比差异不显著（P〉0.05）。隆朋麻醉对犬红细胞免疫黏附性及膜流动性皆存在影响,但对其膜流动性的影响不显著（P〉0.05）。

Study on the effect of Xylazine on erythrocyte adhesion and membrane fluidity in dogs.12 healthy dogs were selected in this experiment,were injected Xylazine 3.0 mg/kg by weight with intramuscular injection,collected the blood 5 mL from peripheral vein at the times of 0（before injected Xylazine） and 2,8,24,72,120,168 h after injected Xylazine,than the blood were centrifugalized to dissociate the erythrocyte,got the erythrocyte suspension and gained the erythrocyte membranes which were detected the C3b acceptor chaplet rate, immunocomplex chaplet rate and erythrocyte fluidity. The results were following：（1）There was a downtrend of the C3b acceptor chaplet rate at 2 h after injecting Xylazine, but the difference with the control group was not significant （P〉0.05）, there was highly significant difference with the control group at 8 h, since then began to restore and returned to normal at 168 h. But the immunecomplex chaplet rate showed the opposite trend, it was very significant higher than the control group at 2 to 24 h from injecting Xylazine, returned to normal at 72 h and there was not significant difference with the control group （P〉0.05）. （2）Deteeted the erythroeyte membrane fluidity by fluorescence polarization, the results showed that membrane fluidity was increased, but there was not significant difference with the control group （P〉0.05）. The conclusions indicated that there were effect of Xylazine anaesthesia on the erythrocyte immunization adhesion and membrane fluidity in dogs, but the influences on membrane fluidity were not significant.