系统性红斑狼疮患者外周血单个核细胞干扰素诱导基因IFIT1和IFIT4 mRNA表达水平的研究

Expressions of Interferon-inducible Genes IFIT1 and IFIT4 mRNA in PBMCs of Patients with Systemic Lupus Erythematosus

为分析系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中两个干扰素(IFN)诱导基因(IFIT1、IFIT4)的表达及其与SLE疾病活动度的相关性,运用实时定量聚合酶链反应(RT-PCR)方法检测95例SLE患者与48名正常对照者的IFIT1、IFIT4的mRNA表达水平,并且与抗ds-DNA抗体等指标比较,分析IFIT1、IFIT4的mRNA表达水平以及与抗ds-DNA抗体、SLEDAI积分之间的相关性。结果显示:①SLE组与正常对照组相比,IFIT1、IFIT4的mRNA表达显著增高(P<0.01);SLE活动组与SLE缓解组比较,IFIT1、IFIT4的mRNA表达增高(P<0.05);SLE组IFIT1、IFIT4的mRNA表达水平之间呈正相关(r>0.5,P<0.05)。②抗ds-DNA抗体与IFIT1、IFIT4的mRNA表达水平及SLEDAI积分呈正相关(r>0.5,P<0.05)。结论:SLE患者IFIT1、IFIT4的mRNA表达水平显著增高,并且与SLEDAI积分呈显著正相关,IFIT1、IF-IT4的mRNA表达水平对SLE患者病情活动程度的判断有较大价值,抑制这2个基因的表达可能为SLE的治疗提供新的靶点。

To investigate the expression levels of interferon-inducible genes （IFIT1, IFIT4） in the peripheral blood mononuelear cells （PBMCs） of patients with systemic lupus erythematosus （SLE）, and the relations between these genes expression levels and disease activity, the expression levels of IFIT1 and IFIT4 mRNA in the 95 patients with SLE and 48 normal controls were detected by Sybr green dye based real-time quantitative PCR method, and these genes expression levels were compared with anti-double strand DNA antibody. The associations between the expression levels of IFIT1, IFIT4 mRNA, anti-double strand DNA antibody and SLEDAI scores in patients with SLE were analyzed. The results showed that the expression levels of IFIT1, IFIT4 mRNA in the SLE patients were significantly higher than those of the normal controls （P 〈 0.01 ）. The expression levels of IFIT1, IFIT4 mRNA in the active SLE patients were higher than those of the inactive SLE patients （P 〈 0.05 ）. The real time expression levels of IFIT1 and IFIT4 mRNA showed positive correlations with each other （P 〈 0.05） in patients with SLE. There was positively correlation between the expression levels of IFIT1, IFIT4 mRNA and the anti-double strand DNA antibody （P 〈 0.05）. The expression levels of IFIT1, IFIT4 mRNA in patients with SLE were significantly higher than those of the normal controls, and positively associated with SLEDAI scores, so they were helpful in evaluating SLE disease activity and severity. To inhibit the expressions of IFIT1, IFIT4 mRNA may provide a novel target for SLE treatment.