窒息新生儿脐血中一氧化氮合酶、生物喋呤、新喋呤变化的临床意义

Clinical Significance of Nitric Oxide Synthase, Biopterin, Neopterin in the Umbilical Blood of Asphyxia Neonates

目的通过观察窒息新生儿血浆一氧化氮合酶（NOS）、生物喋呤（BP）、新喋呤（NP）的变化来了解新生儿窒息及其程度的轻重与这三项指标的相关性，以探讨NOS、BP、NP是否参与窒息的病理生理过程。方法轻度窒息15例，重度窒息15例为病例组，30例同期正常新生儿作为对照组，用固相夹心法酶联免疫吸附试验（ELISA）方法测定血浆中的NOS，高压液相色谱法（HPLC）测定血浆中的BP、NP，同时观察各临床指标的变化。结果重度窒息组血浆NOS、BP、NP均高于轻度窒息组和对照组，差异有高度显著性意义；轻度窒息组血浆NOS、BP、NP均与对照组差异无显著性意义。结论NOS、BP、NP参与了窒息的病理生理过程，可作为新生儿窒息后脑损伤判断的有效指标。

Objective By observing the change of NOS,BP and NP in the plasma of asphyctic newborn infant, we try to check the relativity between the three indicators and existence of neonatal asphyxia as well as its degree of severity so as to investigate whether NOS ,BP and NP partake in the process of pathological and physiological process of asphyxia.Method Taking 15 cases of mild asphyxia, 15 cases of heavy asphyxia newborn infants as a case group;another 30 cases of normal vaginal-delivery babies in same period as control group,we determined NOS by solid-phase sandwich enzyme-linked immunosorbent assay （ELISA） method,and determined BP and NP by high- pressure liquid chromatography （HPLC）.Result With reference to each of three kinds of indicators, plasma, NOS, BP,NP,the values of heavy asphyxia group are higher than the mild asphyxia group and the control group, the differences show high significant; the differences have no significance between mild asphyxia group and the normal control group.Conclusion NOS, BP, NP take part in the process of pathological and physiological process of asphyxia and can be regarded as an effective indicator of brain injury judgment after neonatal asphyxia.