摘要
以不同地理来源的18份不同类型的白菜类作物(Brassica campestris L.)为试材,对其ITS区进行扩增、测序和序列比对,构建NJ系统树,研究白菜类作物ITS序列的变异情况。结果表明:白菜类作物中不同类型材料的ITS序列没有明显变异,序列非常保守;白菜类作物与芸薹属其他种间存在明显变异。芸薹属中的基本种内的变种间没有明显变异;复合种内的变种间有明显变异,分别倾向于禹氏三角(U-triangle)假说推测的父母本。
The ITS sequences of 18 different types of Brassica rapa were amplified by PCR and sequenced,and then the NJ tree was constructed by ITS sequences to analyze the variations of ITS sequence. The results show that the sequences of the ITS of Brassica rapa are very conservative,and no variations exist between different types. Brassica rapa has variations with other species of Brassica respectively. The ITS sequences have variations among the species of Brassica,and the different type materials of the basic species of Brassica have no variations. However, those of the complicate species have variations and the ITS sequences tend to the putative parents of the U-triangle respectively.
出处
《中国蔬菜》
北大核心
2009年第24期13-18,共6页
China Vegetables
基金
"十一五"国家科技支撑计划(2006BAD01A7
2006BAD13B06-4-6)
公益性行业(农业)科研专项(nyhyzx07-007)
农业部蔬菜遗传与生理重点开放实验室项目
关键词
芸薹属
NRDNA
内转录间隔区
遗传距离
Brassica
nrDNA
Internal transcribed spacer （ ITS）
Genetic distance
