摘要
选用2个叶面积大的黄瓜品种(D9320、D2005)和2个叶面积小的黄瓜品种(D0455、D0401)配制杂交组合,筛选叶面积差异较大的亲本组合D0401×D9320构建F2分离群体,采用加性-显性(AD)模型进行遗传分析。结果表明:黄瓜叶面积是由多基因控制的数量性状,以显性效应为主,存在一定的加性效应,狭义遗传力和广义遗传力较高。采用SSR分子标记构建遗传连锁图谱,利用复合区间法对黄瓜叶面积进行QTL定位。结果表明:CSWACC03、CSWCT05BZ和CSCTTT15a位于同一个连锁群上,连锁群总长度为68.3cM;检测出1个与黄瓜叶面积有关的QTL,与最近标记的距离为3.6cM,且变异贡献率为8.65%。
Two large leaf area cucumber （ Cusumis sativus L. ） varieties （ D9320,D2005） and two small leaf area cucumber varieties （ D0455,D0401） were selected to build a hybrid combination. A parental combination with bigger leaf area difference D0401 × D9320 was screened to build up self-isolated groups of F2 generation. Genetic analysis was conducted by using additive-dominance （ AD） model. The results showed that cucumber leaf area was a quantitative trait controlled by multi-genes. It took dominant effect as main character, and there were certain additive effects with higher narrow-sense and broad-sense heritabilily. SSR molecular markers were adopted to build genetic linkage map, using composile interval mapping methods for QTL location of cucumber leaf areas. The results showed that CSWACC03, CSWCT05Bz and CSCTTT15a were located on the same linkage group. The total length of linkage group was 68.3 cM. One QTL was detected in connection with cucumber leaf area. Its distance to the nearest marker was 3.6 cM and the contribution rate to mutation was 8.65 %.
出处
《中国蔬菜》
北大核心
2009年第24期43-46,共4页
China Vegetables
基金
国家“863”计划项目(2006AA100108-4)
黑龙江省科技厅国际合作项目(WB06B14)
