铜对Ctr1基因mRNA表达的影响

Determination of Effect of Copper on the Expression of Ctr1 mRNA

采用半定量RT-PCR法检测铜对猪传代肾细胞(PK15)中特异性铜转运蛋白Ctr1基因mRNA表达水平的影响,以β-actin为内参考,对各试验组cDNA同时进行PCR扩增。结果,猪肾PK15细胞/%1基因mRNA表达量在7.8～31.2μmoL.L-1 Cu范围内随铜添加浓度的升高减少,当铜添加浓度达到62.5μmoL.L-1时,其表达量又有所回升,呈双相反应。

To semiquantitativly determine the effect of copper on Ctr1 mRNA in PK15 cell by reverse transcription-polymerase chain re-action,β-actin was the inner-reference.Samples were amplified with cDNA in the experimental groups.The expression of copper transporter（Ctr1） gene mRNA showed a double-way response to copper ionic concentration in culture media,decreasing with enhancing copper ionic concentration from 7.8 to 31.2 μmoL.L^-1 Cu and increasing with enhancing copper ionic concentration to 62.5 μmoL.L^-1 Cu in culture media.