CZE分析抑肽酶中去丙氨酸-抑肽酶和去丙氨酸-去甘氨酸-抑肽酶

Analysis of Des-Ala-aprotinin and Des-Ala-des-Gly-aprotinin in Aprotinin by Capillary Zone Electrophoresis

目的:建立毛细管区带电泳分析抑肽酶中的去丙氨酸-抑肽酶和去丙氨酸-去甘氨酸-抑肽酶的方法。方法:未涂层石英毛细管柱60 cm×75μm;运行缓冲液120 mmol.L-1的磷酸二氢钾溶液(pH2.5);运行电压为12 KV,柱温30℃;检测波长214 nm。结果:抑肽酶、去丙氨酸-抑肽酶、去丙氨酸-去甘氨酸-抑肽酶迁移时间的RSD分别为1.13%,1.11%,1.11%,校正峰面积(A/t)的RSD分别为3.96%,3.94%,3.84%,A/t%的RSD分别为0.18%、0.96%,1.13%。结论:本法分析抑肽酶中的去丙氨酸-抑肽酶和去丙氨酸-去甘氨酸-抑肽酶高效快速、杂质分离度高、操作成本低,有助于控制其产品质量。

Objective： To establish a capillary zone electrophoresis method for the analysis of des-Ala-aprotinin and des-Ala-des-Gly- aprotinin in aprotinin. Method： A fused silica capillary （60 cm × 75 μm） was used; The running buffer was composed of 120 mmol · L^-1 potassium dihydrogen phosphate （pH 2. 5 ） ; The applied voltage was 12 kV and capillary temperature was 30℃ ; The detection wavelength was 214nm; Result： The RSD of the migration time for aprotinin, des-Ala-aprotinin and des-Ala-des-Gly-aprotinin were 1.13% , 1.11% , 1.11% , corrected peak area were 3.96% , 3.94% , 3.84% , and RSD of A/t% were 0. 18% , 0. 96% , 1.13%. Conclusion： The method was rapid, high in resolution, cheap for the operation, suitable for analyzing des-Ala-aprotinin and des-Alades-Gly-aprotinin in aprotinin, controlling its quality.