转基因鼠前列腺癌模型CT灌注成像及介入治疗初探

CT perfusion imaging and interventional therapy of genetically engineered mouse prostate cancer

目的运用CT灌注成像评价转基因鼠前列腺癌模型生长过程中的血流灌注情况并报告初步介入治疗结果。方法6只转基因鼠前列腺癌模型及正常小鼠5只进行CT灌注成像;1只转基因鼠前列腺癌模型进行介入手术(TAE)。结果肿瘤边缘的BF、BV,大于肿瘤中心和正常前列腺组织,P<0.01,后两者没有明显差异,P>0.05。MTT方面,三者没有明显差异(P>0.05)。PS方面,肿瘤中心大于肿瘤边缘和正常前列腺组织,P<0.01,后两者没有明显差异,P>0.05。1只转基因鼠成功行介入手术(TAE),CT扫描显示碘油沉积在肿瘤内,处死后病理观察肿瘤坏死严重。结论CT灌注成像技术可以用来评价转基因鼠前列腺癌模型的血流动力学变化,介入治疗对前列腺癌具有潜在的临床应用价值。

Objective To evaluate the blood perfusion in tumors of genetically engineered mouse prostate cancer models and report the results of interventional therapy.Methods：CT perfusion was performed in six genetically engineered mouse prostate cancer models and five normal mouse.One genetically engineered mouse prostate cancer model was performed transcatheter arterial chemo-embolization（TACE） with Lipiodo1.Results：（1） The blood flow（BF） and blood volume（BV） value in the peripheral regions of the tumor were lager than those in the center of tumor and normal prostate tissue（P〈0.01）.However,there were no statistic difference between of the BF and BV value in the center of tumor and normal prostate tissue（P〉0.05）.（2） For the permeability surface（PS）,the value in the center of tumor were larger than those in the peripheral regions of the tumor and normal prostate（P〈0.01）.There were no statistic difference between the peripheral regions of the tumor and normal prostate.（P〉0.05）.The interventional therapy was successfully performed in one genetically engineered mouse prostate cancer model.CT scan showed that lipiodol deposited in the tumors.Immunochemistry displayed that the necrosis in the tumors after sacrificed.Conclusion：CT perfusion is suitable to evaluate the change of hemodynamics in the genetically engineered mouse prostate cancer model.There are potential clinical applicational value of TAE in the prostatic carcinoma.