期刊文献+

骨骼肌电穿孔转基因技术研究进展

Advance on Electroporation for Gene Transfer to Skeletal Muscles
下载PDF
导出
摘要 目前,在大动物模型试验中,骨骼肌电穿孔转基因技术已被广泛用于转染一些功能基因,并能显著提高非病毒载体基因的转染效率。笔者就电穿孔促进基因通过细胞膜的可能机理及影响骨骼肌电穿孔转基因效率的关键因素(如物种、细胞直径、场强、脉冲参数、电极和电脉冲仪等)进行了综述。 At the present,electroporation of skeletal muscle has been used extensively to transfer a number of functional genes in multiple large animal models.Electroporation greatly increases transfection efficiency of nonviral-vector gene in skeletal muscle.The possible mechanism was discussed on electro-gene-transfer across the cell membrane and the critical factors were analyzed which had a full impact on skeletal electro-gene-transfer efficacy include species,cell size,field strength,pulse parameters,electrodes and electroporater.
出处 《中国畜牧兽医》 CAS 北大核心 2009年第12期187-191,共5页 China Animal Husbandry & Veterinary Medicine
基金 新疆生产建设兵团院士基金(2007JC19) 新疆农垦科学院引导项目(YYD2009-4)
关键词 体内电穿孔 骨骼肌 质粒 in vivo electroporation skeletal muscle plasmid
  • 引文网络
  • 相关文献

参考文献37

  • 1Aihara H, MiyazakiJ. Gene transfer into muscle byelectroporation invivo[J]. NatureBiotechnology, 1998, 16(9): 867-870.
  • 2Babiuk S, Baca Estrada M E, Foldvari M, et al. Electroporation improves the efficacy of DNA vaccines in large animals[J]. Vaccine, 2002, 20(27-28): 3399-3408.
  • 3Bettan M, Emmanuel F, Darteil R, et al. High level protein secretion into blood circulation after electric pulse mediated gene transfer into skeletal muscle[J]. Molecular Therapy, 2000,2 (3) : 204-210.
  • 4Brown P A, Davis W C, Draghia Akli R. Immune enhancing effects of growth hormone releasing hormone delivered by plasmid injection and electroporation[J]. Molecular Therapy, 2004, 10(4): 644-651.
  • 5Bureau M F, Gehl J, Deleuze V, et al. hnportance of association between permeabilization and electrophoretic forces for intramus cular DNA electrotransfer[J]. Biochirnica Biophysica Acta, 2000, 1474 (3): 353-359.
  • 6Bureau M F, Naiml S, Torero I R, et al. Intramuscular plasmid DNA electrotransfer: biodistribution and degradation [J]. BiochimicaBiophysica Acta, 2004, 1676(2): 138-148.
  • 7Chang D C, Reese T S. Changes in membrane structure induced by electroporation as revealed by rapid-freezing electron microscopy[J]. Biophysical Journal, 1990, 58(1): 1-12.
  • 8Draghia Akli R, Ellis K M, Hill L A, et al. High efficiency growth hormone releasing hormone plasmid vector administration into skeletal muscle mediated by electroporation in pigs[J]. The Federation of American Societies for Experimental Biology, 2003, 17(3): 526-528.
  • 9Draghia Akli R, Fiorotto M L, Hill L A, et al Myogenic expression of an injectable protease resistant growth hormone-releasing hormone augments long-term growth in pigskin. Nature Biotechnology, 1999, 17(12): 1179-1183.
  • 10Draghia-Akli R, Khan A S, Cummings K K, et al. Electrical enhancement of formulated plasmid delivery in animals [J]. Technology in Cancer Research and Treatment, 2002,1 (5) : 365 -371.

二级参考文献2

共引文献5

相关主题

;
使用帮助 返回顶部