摘要
本试验研究建立了猪尿中莱克多巴胺（Ractopamine,RAC）残留的直接竞争化学发光免疫检测方法。合成了莱克多巴胺和辣根过氧化物酶的偶联物（RAC-HRP）,方法的检测限为0.09 ng/mL,空白猪尿中添加浓度为0.5、10和100 ng/mLRAC时,检测范围为0.3～157.0 ng/mL;回收率为76.3%～87.6%,批内变异系数为10.0%～12.2%,批间变异系数为14.2%～15.2%。
A method was developed for the determination of RAC residues in swine urine by direct competitive chemiluminescence immunoassay（CLIA）.The limit of detection was 0.09 ng/mL and the detection ranged from 0.3 ng/mL to 157.0 ng/mL.At the fortified levels of 0.5 ng/mL,10 ng/mL and 100 ng/mL,mean recoveries ranged from 76.3% to 87.6%.The intra-assay variations were 10.0% to 12.2% and the inter-assay variations were 14.2% to 15.2%.
出处
《中国畜牧兽医》
CAS
北大核心
2009年第12期200-203,共4页
China Animal Husbandry & Veterinary Medicine
关键词
化学发光免疫分析
莱克多巴胺
猪尿
chemiluminescence immunoassay
Ractopamine
swine urine
