摘要
目的探讨18氟标记的脱氧葡萄糖(18F-FDG)PET/CT无创检测不稳定斑块的可行性。方法用新西兰雄性大白兔建立动脉粥样硬化模型,注射18F-FDG后行腹主动脉PET/CT成像,离体动脉数码照相,测定动脉片段的放射强度,选取不稳定和稳定斑块各30块,比较其放射强度,采用免疫组化方法观察斑块中的巨噬细胞和平滑肌细胞并计数。结果PET/CT活体成像可见实验组沿腹主动脉走行的不均匀的放射性分布,与离体动脉的斑块分布基本一致;不稳定斑块组的靶-非靶比值和巨噬细胞数明显高于稳定斑块组(P<0.01),而平滑肌细胞数明显降低(P<0.01);靶-非靶比值与斑块中的巨噬细胞数呈正相关(r=0.815,P<0.01),而与平滑肌细胞数呈负相关(r=-0.684,P<0.01)。结论18F-FDGPET/CT无创检测实验性不稳定斑块具有一定的可行性。
Objective To study the feasibility of noninvasive detection of unstable plaques with ^18F-Fluorodeoxyglueose （^18SF-FDG） PET/CT imaging. Methods Atherosclerotic plaques were induced in male New Zealand white rabbits. Animals were injected with FDG labeled with ^18 F, then examined with PET/CT. Aorta was explanted for photography with digital camera,and ^18F-FDG uptake analysis. Thirty unstable plaques and 30 stable plaques were ehoosed so as to compare the quantitativly ^18 F-FDG uptake. The number of macrophages and smooth muscle cells was detected by immunohistochemical technique. Results Experimental group showed inconsistent uptake of ^18F-FDG in the abdominal aorta. The results were confirmed in the ex vivo digital photo of the explanted aorta. The target to non target ratio（T/NT） and maerophages of unstable plaques were higher than stable plaques（ P 〈 0. 01 ） , but smooth muscle cells obviously reduced （P 〈0. 01 ）. Correlation analysis showed that there was a positive corre- lation between T/NT and macrophage content（ r = 0. 815 ,P 〈 0.01 ）, and a negative correlation between T/NT and SMC content（r = -0. 684,P 〈 0.01 ）. Conclusion ^18F-FDG PET/CT can constitute an attractive imaging method for the noninvasive detection of experimental unstable plaques.
出处
《基础医学与临床》
CSCD
北大核心
2010年第1期33-36,共4页
Basic and Clinical Medicine
基金
北京市教委基金(KM200710025006)
