兔吸入一氧化氮毒副反应的实验研究

The Side-effects of No Aspiration in Rabbits

目的：探讨自研一氧化氮（NO）吸入装置使受试兔吸入不同浓度NO的毒副反应。方法：用自研B－951型NO吸入装置，使受试兔12周内间断吸入25、50、100ppm的NO，对照组吸正常空气；吸气前后，抽血查：ALT、BUN、Cr、MetHb值及血常规；第12周活杀兔子解剖，观察重要脏器及组织病理变化。结果：吸气后与吸气前比较，肝肾功能、血常规无明显变化，均在正常范围内；吸NO的各组MetHb值略有升高趋势；组织病理无明显改变。结论：兔在12周内间断（共15小时）吸入100ppm以下的NO，未见明显的生理、生化及组织病理的毒副反应。

Objective : The side -effects of various concentrations of NO aspirated thourgh a designed apparatus in rabbits was investigated. Methods: Rabbits were subjected to 25,50, and 100 ppm NO gas treatment during a 12-week period. Those rabbits aspirated atmosphere served as control. An apparatus with microprocessor-interfaced controller was designed and usedfor the aspiration in the investigation. Before and after treatment, ALT, Cr, BUN, MetHb and CBC were monitored. At the end of the investigation , the rabbits were sacrificed and the major organs were histologically examined. Results: There were no significant changes in liver and renal function, CBC, and histological profile of major organs in the rabbits with NO treatment.while a slight increase was observed in MetHb in the rabbits treated with NO at all concentrations used in the study. Conclusion:There were no significant side -effects on the liver, kidneys ,and lungs in the rabbits received logn-term NO aspiration at concentration up to 100 ppm