血府逐瘀汤影响内皮祖细胞分化的实验研究

Experimental Study on Effects of Xuefu Zhuyu Decoction on Endothelial Progenitor Cells Differentiation

目的:观察血府逐瘀汤对骨髓内皮祖细胞分化的影响。方法:采用不同浓度的血府逐瘀汤含药血清和空白对照血清诱导处理EPCs后,观察药物对EPCs吞噬乙酰化低密度脂蛋白、分泌NO和细胞超微结构的影响。结果:经含药血清诱导48h,15%含药血清能显著提高内皮祖细胞吞噬乙酰化低密度脂蛋白和分泌NO的能力,且透射电镜观察表明药物有利于内皮祖细胞的分化,在5%和10%的含药血清组可观察到内皮细胞的特征性结构。结论:血府逐瘀汤具有诱导内皮祖细胞分化成内皮细胞的作用。

Objective： To observe the effect on differentiation of EPCs by Xuefu Zhuyu Decoction. Methods： To observe EPCs on uptake of ac-LDL, secretion NO and cell structure affected by Xuefu Zhuyu Decoction-contained serum. Results： Compared to the control group, induced by 15% Xuefu Zhuyu Decoction-contained serum （XFZYDCS） for 48h could improve the EPCs for uptake ac-LDL and secretion NO; In 5% and 10% XFZYDCS groups, Weible-Palade body, typical endothelial cells structure, could be observed by electronic telescope. Conclusion： Xuefu Zhuyu Decoction could induce EPCs differentiate into endothelial cells.