摘要
运用3种方法（C8反相柱层析．复性法、多步层析法和本实验室改进的c8反相柱层析．非复性法）去除重组HuMIF蛋白的内毒素，比较其所得蛋白内毒素含量、酶活性以及蛋白回收率等，评价这3种去内毒素方法的优劣。结果表明，c8反相柱层析．复性法蛋白回收率很低，酶活性低但内毒素去除效果好；多步层析法蛋白回收率高，酶活性高但内毒素去除效果稍差；而本实验室改进的c8反相柱层析．非复性法蛋白回收率稍差，酶活性较好且内毒素去除效果不错。由于c8反相柱层析一非复性法内毒素去除方法简单有效，适合实验室范围内推广。
Three different protein endotoxin removal methods including C8 reversed-phase column chromatography-renaturation, Multi-step chromatography and an improved C8 reversed-phase column chromatography-non-renaturation were compared in present paper. The evaluation items included endotoxin protein content, enzyme activity and protein recovery. The results showed that the C8 reversed-phase column chromatography-renaturation may get very low protein recovery rate, low protein activity but good endotoxin removal efficiency; multi-step chromatography may get high protein recovery rate, high protein activity and less endotoxin removal efficiency; while reversed-phase column chromatography-non-renaturation may get low protein recovery rate, relatively high protein activity and good endotoxin removal efficiency. As a result, it seems that C8 reversed-phase column chromatography-non- renaturation method might be suitable for laboratory application and dissemination for its simple handling and effectiveness.
出处
《寄生虫与医学昆虫学报》
CAS
2009年第4期250-254,共5页
Acta Parasitologica et Medica Entomologica Sinica
基金
基金项目：中国自然科学基金项目（No.30700761）
国家重点基础研究发展计划（973）项目（#2007CB513100）
关键词
巨噬细胞迁移抑制因子
内毒素
蛋白回收率
酶活性
Macrophage migration inhibitory factor
Endotoxin
Protein recovery rate
Enzymatic activity
