鼻腔AChR耐受低调EAMG大鼠特异性T淋巴细胞反应

The downregulation of specific T Lymphocyte responses by nasal administration of AChR in EAMG rats

用乙酰胆碱受体(AChR)加完全弗氏佐剂(CFA)免疫前,鼻腔给予大鼠AChR可有效地预防实验性自身免疫性重症肌无力(EAMG)。本文结果表明,与非耐受对照组相比,鼻腔AChR耐受组免疫后,腘窝、腹股沟淋巴结(PILN)的单个核细胞(MNC)AChR特异的淋巴细胞增生反应受到明显抑制,鼻腔耐受组PILN中AChR特异的CD4^+/CD8^-克隆增生亦明显受抑制,差异均有显著性。此外,鼻腔耐受组AChR特异性皮肤迟发型过敏反应(DTH)也显著降低。提示鼻腔AChR耐受后引起的特异性T淋巴细胞反应的低调可能是临床肌无力抑制的基础。

Nasal administration of acetylcholine receptor (AChR) to experimental animals prior to immunization with AChR plus complete Freund's adjuvant (CFA) can effectively prevent clinical signs of experimental autoimmune myasthenia gravis(EAMG). In the present report we demonstrate that the suppression of AChR - specific lymphocyte proliferation responses from mononuclear cells (MNC) of popliteal and inguinal lymph nodes (PILN) in nasal AChR tolerized rats were more obvions than that in non-tolerized EAMG control rats after immunization;the suppression of AChR - specific CD4+/CD8- clonal proliferation from PILN of nasally tolerized rats were obvious too,the statistically significant differences were found as compared to the non-tolerized control rats;the AChR-specific skin delayed - type hypersensitivity (DTH) responses were markedly decreased in nasally tolerized rats. The results suggest the downregulation of specific T 1gmp hocgte responses induced by nasal administration of AChR may be the foundation of clinical myasthenia suppression.