阴道念珠菌菌株的分型和相似性分析

Classification and Analogous Analysis of Candida Strains Isolated from Vulvovaginal Candidiasis

目的 探讨阴道念珠菌病发病与念珠菌菌株的关系。方法 选择两组白念珠菌 ,A组来源于健康妇女阴道分泌物 ,B组来源于阴道念珠菌病患者。选用自动微生物诊断鉴别系统和氨基黑染色法两种表型识别技术检测受检菌株生化反应及蛋白酶分泌参数 ,进而用数值分类技术行相似性分析。结果 A、B两组间福寿草醇存在显著性差异 (P <0 0 5 ) ,也进一步证实蛋白酶的分泌能力在两组间存在显著性差异 (P <0 0 1)。 5 6株白念珠菌共产生 41型 ,可大致分为 7类 ,c、f和g类以B组分离株为主 ,b和d类以A组分离株为主。结论 福寿草醇和蛋白酶的升高可能与阴道念珠菌病发病相关。数值分类技术在菌株相似性分析时 ,有其优越性 ,有待进一步总结。

Objective To study the correlation between Candida strains and vulvovaginal candidiasis. Methods Two groups of Candida albicans strains were chosen. Strains of group A and group B were isolated from vaginal discharge of normal women and of patients with candidal vaginitis respectively. The two methods, AMS (automated microbiological detection and identification system) and amino black stain, were applied to detect two phenotypic parameters, biochemical reaction of the strains and secretory capacity of the proteinases respectively, and analogous analysis was performed then. Results There was a significant difference in the levels of adonitol (P<0 05) between group A and B, and further study proved that the difference in the secretory capacity of proteinase was significant between them (P<0 01). By using the method of analogous analysis, 56 strains of Candida albicans were classified into 41 types, and could be approximately divided into 7 sub groups. The strains in the sub group c, f and g were mainly in group B and the sub group b and d mainly in group A. Conclusion Increase in the levels of adonitol and proteinase may be related to the development of vulvovaginal candidiasis. At the same time, the advantage of using numerical taxonmic technique in stratin analysis is discussed.